CELL BIOLOGY OF BACTERIAL INFECTIONS

 

UMR 9004, IRIM

Institut de Recherche en Infectiologie de Montpellier

 

 

  • Organismes de tutelle : CNRS – Université de Montpellier
  • DU : Jean-Michel MESNARD (04 34 35 94 01)
  • Site webhttp://www.irim.cnrs.fr/index.php
  • Centre : Université de Montpellier – Faculté des Sciences
  • Localisation : 919 Route de Mende – 34293 Montpellier Cedex 05
  • Tél : 04 34 35 94 01
  • Fax : 04 34 35 94 11
  • Contact site IMH : N/A

 

Animateur(s)

〉 Matteo BONAZZI (CR1 CNRS) 04 34 35 94 59

Composition équipe

〉  1 Chercheur, 2 post-doc, 1 ingenieur, 1 PhD student

 

Thématique

Cellules infectées par : Coxiella qui se réplique dans un des compartiments / LAMP1-positive

Our aim is to identify and characterize the Coxiella factors involved in the key steps of its intracellular replicative cycle: a) adhesion and entry, b) PV biogenesis, c) cell-to-cell spread and d) protection of the host cell from apoptosis. To this aim, we are generating the first bank of Coxiella GFP-tagged mutants by transposon mutagenesis that are being screened using novel and robust imaging-based high content screening (HCS) assays. Our approach has recently led to the identification of Coxiella factors possibly involved in bacterial entry, replication and cell-to-cell spread. We are currently conducting a number of studies to validate and characterize the role of these bacterial candidate proteins.

[<< Cellules infectées par : Coxiella qui se réplique dans un des compartiments / LAMP1-positive]

 

 

Couple microorganisme-hôte

Coxiella burnetii/cellules eucaryotes

 

Organismes étudiés

Coxiella

 

Mots clés techniques

high-content screening, mutagenèse, RNAi

 

Travaux de terrain

Non

 

Outils communs

N/A

 

Thèses en cours

Characterization of 5 candidate effector proteins of the zoonotic bacterium Coxiella burnetii and their role in the subversion of host cell functions

The aim of this project is to validate the identified candidates as proper Coxiella effectors, further investigate the phenotypes associated with their mutation and identify their host-cell targets to understand their mode of action. This study will reveal important mechanisms of Coxiella infections and potentially highlight bacterial and/or host targets to develop specific antibacterial treatments against Q fever.

 

Principales publications

1: Martinez E, Cantet F, Bonazzi M. Generation and multi-phenotypic high-content screening of Coxiella burnetii transposon mutants. J Vis Exp. 2015 May 13;(99):e52851. doi: 10.3791/52851. PubMed PMID: 25992686; PubMed Central PMCID: PMC4542693.

2: Duron O, Noël V, McCoy KD, Bonazzi M, et al. The Recent Evolution of a Maternally-Inherited Endosymbiont of Ticks Led to the Emergence of the Q Fever Pathogen, Coxiella burnetii. PLoS Pathog. 2015 May 15;11(5):e1004892. doi: 10.1371/journal.ppat.1004892. eCollection 2015 May. PubMed PMID: 25978383; PubMed Central PMCID: PMC4433120.

3: Norville IH, Hartley MG, Martinez E, Cantet F, Bonazzi M, Atkins TP. Galleria mellonella as an alternative model of Coxiella burnetii infection. Microbiology. 2014 Jun;160(Pt 6):1175-81. doi: 10.1099/mic.0.077230-0. Epub 2014 Mar 27. PubMed PMID: 24677067.

4: Martinez E, Cantet F, Fava L, Norville I, Bonazzi M. Identification of OmpA, a Coxiella burnetii protein involved in host cell invasion, by multi-phenotypic high-content screening. PLoS Pathog. 2014 Mar 20;10(3):e1004013. doi: 10.1371/journal.ppat.1004013. eCollection 2014 Mar. PubMed PMID: 24651569; PubMed Central PMCID: PMC39613

 

Collaborations

N/A